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Clin Shoulder Elbow > Volume 17(2); 2014 > Article
Clinics in Shoulder and Elbow 2014;17(2):64-67.
DOI: https://doi.org/10.5397/CiSE.2014.17.2.64    Published online March 31, 2014.
Changes in Matrix Metalloproteinase and Tissue Inhibitors of Metalloproteinase in Patients with Rotator Cuff Tears
Oh Soo Kwon, Young Yul Kim, Ji Yoon Ha, Han Bit Kang
Department of Orthopedic Surgery, The Catholic University of Korea, Daejeon St. Mary's Hospital, Daejeon, Korea. oskn@catholic.ac.kr
Received: 25 February 2014   • Revised: 20 March 2014   • Accepted: 30 April 2014
Abstract
BACKGROUND: The purpose of this study was to determine whether in patients with rotator cuff tears a correlation exists between molecular changes and clinical parameters such as age, duration of symptom, range of motion, and tear size. Molecular changes of matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP) were assessed by measuring messenger RNA (mRNA) levels of the two proteins.
METHODS
The rotator cuff tissue from was obtained from the edge of a torn tendon revealed after debridement by a motorized shaver. Using the sample of rotator cuff tissue, the reverse transcription polymerase chain reaction was performed to quantify MMP-2 and TIMP-2 mRNA expression. To determine whether mRNA levels and the clinical variables, such as age, defect size, range of motion (ROM) of shoulder, and duration of symptoms, show any correlation, Spearman's correlation coefficients were used to test for significant differences.
RESULTS
There was an inverse correlation between the mRNA levels of MMP-2 and TIMP-2 from the torn rotator cuff tendons regardless of the clinical variables. However, comparison of mRNA levels versus clinical parameters such as age, defect size, range of motion and duration of symptoms revealed a number of findings. We found a significant correlation between age and mRNA levels of MMP-2 from torn cuffs (r = 0.513, p = 0.021). Further, we found a significant correlation between defect size in the full thickness tears and mRNA levels of MMP-2 (r = 0.454, p = 0.045). Conversely, no significant association between mRNA levels of MMP-2 and ROM or duration of symptom was found.
CONCLUSIONS
Our results suggest that both MMP-2 and TIMP-2 may be involved in the disease process of rotator cuff tears. Although the level of mRNA expression of MMP-2 and TMP-2 remain constant in torn rotator cuffs irrespective of the clinical variables, their levels may be influenced by age and defect size, which could account to change in tendon degradation and the healing process.
Key Words: Rotator cuff; Matrix metalloproteinase; Tissue inhibitor of metalloproteinase, Polymerase chain reaction


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