The role of vitamin D in shoulder health: a comprehensive review of its impact on rotator cuff tears and surgical results

Article information

Clin Shoulder Elb. 2025;28(1):93-102

Publication date (electronic) : 2024 July 30

doi : https://doi.org/10.5397/cise.2024.00220

Mohammad Daher^,¹

, Oscar Covarrubias ², Ryan Lopez ¹

, Peter Boufadel ¹, Maria Catherine Rita Hachem ³, Ziad Zalaquett ³, Mohamad Y. Fares ¹

, Joseph A. Abboud ¹

¹Division of Shoulder and Elbow, Rothman Orthopaedic Institute, Philadelphia, PA, USA

²Department of Orthopedics, Brown University, Providence, RI, USA

³Department of Orthopedics, Hotel Dieu de France, Beirut, Lebanon

Corresponding Author: Mohammad Daher Division of Shoulder and Elbow, Rothman Orthopaedic Institute, 925 Chestnut St, Philadelphia, PA 19107, USA Tel: +1-26-7465-8920, E-mail: mohdaher1@hotmail.com

Received 2024 March 7; Revised 2024 May 15; Accepted 2024 May 18.

Abstract

Vitamin D deficiency is highly prevalent in the general population and is associated with various chronic health conditions. In addition to its role in bone mineralization, Vitamin D has various physiological effects that may impact the pathogenesis of shoulder pathologies. Vitamin D deficiency may also affect outcomes after shoulder surgeries, such as rotator cuff repair and total shoulder arthroplasty. Vitamin D plays a role in tissue healing, bone growth, and maintenance of homeostasis in skeletal muscle cells. Vitamin D also has anti-inflammatory effects that are important to rotator cuff health. Vitamin D deficiency is highly prevalent in patients with rotator cuff tears, suggesting its role as a potential risk factor. Vitamin D deficiency has been associated with decreased preoperative shoulder strength as well as increased retear rates, postoperative stiffness, and the need for revision surgery in patients who underwent rotator cuff repair. Studies have also demonstrated a potential association between vitamin D deficiency and increased risk of revision after total shoulder arthroplasty. Further research is necessary to elucidate the direct role of vitamin D in the pathogenesis of rotator cuff tears and its impact on clinical outcomes after rotator cuff surgery and total shoulder arthroplasty.

Keywords: Rotator cuff tears; Vitamin D

INTRODUCTION

Vitamin D is essential for the homeostatic regulation of calcium involved in a wide range of physiological processes, such as bone mineralization, nerve transmission, blood clotting, hormone secretion, and muscle contraction [1,2]. Vitamin D deficiency has been associated with chronic health conditions and is estimated to affect one billion people worldwide. Vitamin D deficiency prevalence ranges from 8.3% to 71% in patients with rotator cuff tears (RCTs) [3-5]. Of particular concern to the orthopedic surgeon is age-related bone loss thought to occur secondary to dysregulation of vitamin D metabolism with an associated decrease in intestinal calcium absorption and increased catabolism of the active form of vitamin D (1,25-dihydroxyvitamin D) [1]. Studies in lower extremity total joint arthroplasty patients have found associations between preoperative vitamin D deficiency and persistent postoperative pain, decreased patient-reported outcome measures, and increased risk of periprosthetic joint infections (PJIs) [6-9]. The impact of preoperative vitamin D deficiency on outcomes of upper extremity surgery has been less thoroughly characterized. The link between vitamin D and osteoporosis has led to the hypothesis that low vitamin D level (<30 ng/L) impacts shoulder surgery outcomes.

Due to age-related tendon and cartilage degeneration, elderly patients often undergo rotator cuff repair (RCR) and total shoulder arthroplasty (TSA). These procedures can be complicated by age-related bone loss as such patients rely on dense cancellous bone for durable fixation of suture anchors and humeral head components [10,11]. As a result of its involvement in a broad range of homeostatic processes from muscle metabolism to immune regulation, vitamin D deficiency may affect outcomes of shoulder surgery through systemic effects other than just bone mineralization [12-14]. In fact, studies have shown that vitamin D is implicated in healing modulation and muscle homeostasis, impacting the rotator cuff muscles. Therefore, the purpose of this narrative review is to describe the impact of hypovitaminosis D on outcomes of RCR and TSA.

DATA COLLECTION

PubMed, Cochrane, and Google Scholar (page 1–20) searches were performed to December 2023 in search of qualifying papers. Using Boolean Operators, a combination of the keywords “rotator cuff” AND “vitamin” was used. Reference lists from papers and online searches were also used to find relevant literature. The data were extracted by one researcher, and the article selection was verified by a different researcher.

PATHOPHYSIOLOGY OF HYPOVITAMINOSIS D ON THE ROTATOR CUFF (Fig. 1)

Fig. 1.

Illustration of the effects of vitamin D on healing (A), inflammation (B), and muscle (C) homeostasis. TNF: tumor necrosis factor, NF-κB: nuclear factor kappa B, MMP: matrix metalloproteinases, IL: interleukin, CRP: C-reactive protein. Made with BioRender.

Vitamin D plays an important role in various physiological processes, particularly in bone growth, healing, and maintenance of homeostasis in skeletal muscle cells. Vitamin D is metabolized to its active form after the hepatically modified calcifediol (25-hydroxyvitamin D3) is hydroxylated in the kidney to calcitriol (1,25-dihydroxyvitamin D3). Vitamin D exerts its effects by interacting with vitamin D receptors (VDRs) to play a role in a variety of functions including, but not limited to, bone mineralization, regulation of blood calcium and phosphate levels, regulation of parathyroid hormone, and modulation of inflammatory biomarkers.

Modulation of Tissue Healing

After RCR, tendon healing at the tendon-bone interface results in deposition of fibrovascular scar tissue that is weaker than the native tendon and is more susceptible to failure [15]. This new tissue is formed of collagen type III and is later replaced by type I collagen. The ratio of type I to type III collagen is, therefore, an indicator of healing after RCR [15,16]. This collagen architecture is mediated by remodeling by matrix metalloproteinases (MMP), specifically MMP-1, MMP-9, and MMP-13 [17-21]. Research has revealed a role for vitamin D in tissue healing. One pathway by which vitamin D modulates healing is the downregulation of cellular response to tumor necrosis factor (TNF)-α by inhibition of c-Jun-N-terminal kinase. This blocks both nuclear factor kappa B and activator protein 1 transcription factors [22]. These actions collectively downregulate MMP-9, a factor associated with compromised healing of the rotator cuff when overexpressed [22]. MMP-9 expression is increased in the tendon-to-bone healing site in rats with vitamin D deficiency [12].

Vitamin D as an Anti-inflammatory Agent

The anti-inflammatory effects of vitamin D have been elucidated in several studies. Insufficient vitamin D level has been linked to higher levels of inflammatory markers like TNF-α, interleukin (IL)-1β, IL-5, IL-6, IL-13, and C-reactive protein [23-27]. The association with anti-inflammation is further supported by Kim et al. [23], who found increased expression of inflammatory genes (IL-6, IL-1B) and gene products (IL-6) in the deltoid and supraspinatus tissues of RCT patients with vitamin D deficiency.

Muscle Homeostasis

Vitamin D has been demonstrated to play a role in healthy muscle growth and metabolism. Deficiencies in vitamin D cause muscle weakness in proximal muscle groups, notably type II (fast-twitch) fibers [13]. Importantly, vitamin D supplementation has a beneficial effect on muscle volume and may reverse vitamin D deficiency-related weakness [13,28,29]. These results have been supported in animal models, in which rotator cuff muscles in vitamin D-deficient rats had inferior biomechanical properties and decreased collagen fiber organization in the enthesis [12].

Adequate VDR expression is crucial for vitamin D to promote myogenesis, proper calcium regulation, and maintenance of muscle homeostasis [25,30]. The expression of VDR is related to vitamin D level [31]. Long-term vitamin D deficiency leads to low expression of VDR, resulting in decreased myocyte protein content, oxidative stress, mitochondrial dysfunction, and lower muscle fiber cross-sectional area [30]). These results were mirrored in VDR knockdown mice [32]. In a study by Rhee et al. [4], vitamin D deficiency in RCR patients led to decreased expression of VDR and myoD and increased atrogin expression in the ipsilateral deltoid muscle. These gene expression changes are consistent with impaired muscle homeostasis and increased muscle atrophy. The torn rotator cuff tendons in this study did not exhibit the same gene expression changes as the deltoid, indicating that unloading of the rotator cuff may alter the muscle’s response to vitamin D deficiency [23]. Increased atrogin expression is implicated in protein degradation via the Src-ERK1/2-Akt-FOXO pathway in myocytes, leading to muscle atrophy [33]. Notably, Kim et al. [23] did not observe an effect of vitamin D deficiency on the expression of adipogenic genes (C/EBPa, PPARgamma) in the deltoid or supraspinatus muscle. These findings correlate with the lack of a relationship between vitamin D deficiency and fatty atrophy observed in the study by Rhee et al. [4].

Vitamin D is important for healing, anti-inflammation, and skeletal muscle homeostasis. In tendon healing after RCR, vitamin D helps regulate collagen formation and modulates tissue repair by downregulating inflammatory responses and MMPs. Deficiency in vitamin D leads to higher levels of inflammatory markers and compromised tendon-to-bone healing. Additionally, vitamin D is essential for muscle growth and metabolism; deficiencies cause muscle weakness and atrophy due to decreased VDR expression and impaired myocyte function. This deficiency alters muscle gene expression, notably increasing atrophy markers that can disrupt muscle homeostasis and regeneration.

HYPOVITAMINOSIS D AND THE ROTATOR CUFF (Table 1)

Table 1.

Summary of the studies assessing the impact of hypovitaminosis D on the rotator cuff

Vitamin D Status as a Risk Factor for RCT

Vitamin D is a key mediator of muscle, tendon, and bone health and has implications in the development of RCTs [23]. In a matched-cohort study of more than 330,000 patients sourced from a large insurance claims database, Albright et al. [34] showed that patients diagnosed with a full-thickness RCT were more likely to have had a vitamin D deficiency diagnosis in the prior 2 years (odds ratio [OR], 2.36). This effect was magnified in women between the ages of 70 and 89 years and diminished in men 30–49 years old [34]. In osteoporotic patients, Liu et al. [35] found that low serum vitamin D was an independent risk factor for RCT.

In RCT patients, the prevalence of insufficient (20–29 ng/mL) or deficient (<20 ng/mL) serum vitamin D has been reported. In a retrospective study of RCR patients by Wilde et al. [36], 36% were identified as having insufficient or deficient serum vitamin D; and Lee et al. [5] reported a low serum vitamin D level in 71% of their RCT patients. In their study of 36 RCT patients undergoing RCR, Rhee et al. [4] reported that only 8.3% of patients had a sufficient vitamin D level.

Radiographic and Histologic Features

The effect of preoperative vitamin D level on radiographic features of RCTs has been investigated in several studies, with mixed results. Rhee et al. [4] performed a study of 36 patients undergoing RCR, revealing no discernable connection between preoperative serum vitamin D level and tear size, degree of retraction, or Goutallier grade of fatty atrophy of the rotator cuff. These findings are consistent with the results of Ryu et al. [37] and Chen et al. [4,38]. These findings are, however, inconsistent with those of Oh et al. [13], who reported significant correlations between lower serum vitamin D level and greater fatty atrophy of the supraspinatus and infraspinatus but not of the subscapularis. Histologically, Rhee et al. [4] demonstrated that, although serum vitamin D level appears to have no effect, tissue vitamin D level correlates significantly with individual muscle fiber cross-sectional area.

Clinical Presentation: Physical Exam & Preoperative Function Scores

Several studies have addressed the impact of vitamin D level on the clinical symptoms of RCTs. In the study conducted by Rhee et al. [4], serum vitamin D level did not correlate with preoperative functional scores; however, the vitamin D level in tissue from supraspinatus biopsies correlated directly with preoperative American Shoulder and Elbow Surgeons (ASES) and Constant scores and inversely with preoperative pain visual analog scale score.

Serum vitamin D level may influence preoperative rotator cuff strength in RCT patients. Oh et al. [13] observed that lower serum vitamin D levels were associated with significantly reduced force generation in both abduction and external rotation. These findings partially align with those of Rhee et al. [4], who also found decreased shoulder abduction strength with lower serum and tissue vitamin D levels. However, they found that tissue vitamin D level impacted internal rotation strength rather than external rotation strength [4].

Vitamin D plays a significant role in muscle, tendon, and bone health, influencing the development and severity of RCTs. Vitamin D deficiency is prevalent among patients with RCT. While preoperative serum vitamin D level does not consistently correlate with radiographic features of RCTs, these levels do affect clinical symptoms and muscle strength. Lower serum vitamin D level is linked to reduced shoulder strength and worse preoperative functional scores, and tissue vitamin D level specifically correlates with muscle fiber area and functional scores.

HYPOVITAMINOSIS D AND RCR (Table 2)

Table 2.

Summary of the studies assessing the impact of hypovitaminosis D on the outcomes of rotator cuff repair

The impacts of hypovitaminosis D and RCR in the clinical setting remain controversial. Angeline et al. [12] demonstrated in rat models that diet-induced vitamin D deficiency affected the early period of healing after RCR, resulting in decreased strength at 2 weeks. This deficiency resulted in reduced formation of fibrocartilage and inferior collagen organization. However, strength was normal four weeks postoperatively despite the persistence of histological abnormalities [12]. As for hypovitaminosis D in humans, Harada et al. [39] studied data from patients in a national claims database and reported that vitamin D deficiency was associated with a higher risk of revision RCR and postoperative stiffness requiring manipulation under anesthesia. Revision surgery was explained to be the result of disrupted healing [39]. However, postoperative stiffness was due to the upregulation of inflammatory cytokines leading to myofibroblast-induced capsular hyperplasia and fibrosis, highlighting the role of vitamin D in inflammatory regulation and myofibroblast activity inhibition [40,41]. Other studies reported similar findings in regard to the increased rate of RCR revision [38,42,43]. Cancienne et al. [42] showed that the difference in the absolute rate of revision RCR was minimal between the two groups and was unlikely to be clinically impactful. In addition, Chen et al. [38] showed that vitamin D deficiency was associated with a higher pain level in the early postoperative period after RCR. Pain was negatively correlated with vitamin D level at 1 and 3 months but not at 6 and 12 months postoperatively [44]. This was explained by the impact of vitamin D on the reduction of cytokines and prostaglandins as well as on T-cell responses [45-49]. Furthermore, Chen et al. [44] showed a statistically significant difference in the Sugaya retear grade depending on the presence or absence of vitamin D deficiency. This conclusion was justified by the effect of vitamin D on the healing structure of the rotator cuff after RCR. Another important finding was the correlation between serum vitamin D level and tissue vitamin D level [4]. Deficiency in both reduced the strength in abduction, but only a low tissue vitamin D level affected external rotation strength [4]. Moreover, the muscle fiber cross-sectional area, which affected muscle strength, was lower in patients with low vitamin D level in the tissue but did not correlate with vitamin D level in the serum. This suggests that vitamin D level in the tissue is more reflective of the impact of vitamin D in the rotator cuff than is the serum level [4].

However, vitamin D deficiency did not affect any of the clinical outcome scores such as the University of California Los Angeles (UCLA) score, ASES score, and the Constant-Murley score [38,44]. Chen et al. [44] reported no impact of vitamin D deficiency on the retear rate after RCR. This was in agreement with the findings of both Ryu et al. [37] and Rhee et al. [4]. Nevertheless, in a cost-effect analysis study, Patel et al. [50] analyzed the cost-effectiveness of pre-operative vitamin D supplementation and reported that non-selective vitamin D supplementation resulted in a mean cost savings of $11,584,742, which was higher than selective vitamin D supplementation ($6,099,341). Therefore, vitamin D supplementation without prior screening is recommended prior to RCR regardless of deficiency.

HYPOVITAMINOSIS D AND TSA (Table 3)

Table 3.

Studies reporting on vitamin D and total shoulder arthroplasty

High rates of vitamin D deficiency have been reported in patients undergoing TSA. In a retrospective review of 218 TSA patients, Inkrott et al. [2] found that 43% had vitamin D insufficiency (<30 ng/mL) and 11% were deficient (<20 ng/mL). Identifying and addressing vitamin D deficiency through supplementation of vitamin D and calcium could allow preoperative optimization of bone mineral density (BMD) given the association of decreased BMD with acromial stress fractures after reverse shoulder arthroplasty [51]. Currently, no studies have specifically examined the impact of vitamin D level on the incidence of stress fractures following reverse shoulder arthroplasty.

Hypovitaminosis D has been associated with increased risk of revision shoulder arthroplasty. In a retrospective cohort study using the PearlDiver database, Smith et al. [3] compared 1,644 TSA patients diagnosed with vitamin D deficiency to 5,022 matched controls. After adjusting for anti-osteoporotic medication prescriptions and osteoporosis diagnoses, the researchers found a significantly higher rate of revision arthroplasty in the vitamin D-deficient group (2.3% vs. 0.8%; OR, 3.3; P<0.001). However, the group was unable to determine the reason for the increased revision rates as there were no significant differences in the rates of implant loosening, periprosthetic fracture, or periprosthetic infection [3]. One potential explanation is a higher rate of secondary rotator cuff insufficiency, which is among the three most common complications of TSA requiring revision [52]. In a retrospective study of 518 TSA patients, Young et al. [53] found that preoperative infraspinatus fatty infiltration was correlated with secondary rotator cuff insufficiency. Considering that low vitamin D level has been identified as an independent predictor of fatty infiltration of rotator cuff muscles in animal studies [13], secondary rotator cuff insufficiency may be an explanation for the significantly higher revision rate in the vitamin D-deficient group. However, the underlying causes of the increased revision rate remain unclear and underscore the need for further research on the impact of vitamin D levels and TSA outcomes.

Vitamin D modulates innate and adaptive immune cell function, suggesting a role in PJI. In a retrospective study of 109 joint arthroplasty (hip, knee, shoulder) patients, Maier et al. [54] found that PJI patients had significantly lower levels of 25-hydroxyvitamin D compared to those undergoing primary arthroplasty or those presenting with aseptic loosening. The most commonly isolated bacterium in PJI of shoulder arthroplasties is the indolent low virulence Cutibacterium acnes [55,56]. However, there were no reported cases of C. acnes infection in the study by Maier et al. [54], with most TSA infections due to Staphylococcus aureus, which limits the generalizability of these findings. In their retrospective PearlDiver study, Smith et al. [3] found no association between PJI and vitamin D deficiency; however, these researchers did find higher rates of urinary tract infections (OR, 1.31; P=0.003) in vitamin D-deficient patients. However, their study was based on PearlDiver, and the PJI diagnosis relied upon accurate coding within the utilized database using International Classification of Diseases, 9th Revision (ICD-9) and 10th Revision (ICD-10) codes. This decreased the reliability of their findings. In addition, the preponderance of research evaluating the relationship between vitamin D level and PJI in TSA patients has inconclusive findings. This highlights the need for further studies with more rigorous methodologies focusing on isolated cohorts of TSA patients to better understand and clarify this association.

MANAGEMENT OF VITAMIN D DEFICIENCY

Serum calcium and 25-hydroxyvitamin D levels should be assessed in the laboratory. PTH level assessment to rule out secondary hyperparathyroidism is also indicated [57]. With a half-life of 2 to 3 weeks, 25-hydroxyvitamin D is the recommended clinical indicator of vitamin D deficiency. Finding more underlying metabolic problems may be aided, if appropriate, by a referral to a metabolic bone specialist [57]. As for oral vitamin D supplementation in the context of hypovitaminosis D, there is no established protocol [57]. The majority of randomized and observational studies has recommended 400–1,000 IU of vitamin D daily [57]. Daily doses of supplementation are thought to be preferable to massive annual dosages, and vitamin D3 is more effective than vitamin D2 in sustaining levels over time [57]. Considering the lack of consensus regarding the ideal therapeutic dosage and the infrequency of vitamin D toxicity, a target 25-hydroxyvitamin D level of 30–40 ng/mL is often advised [57]. However, new recommendations from Osteoporosis Canada and the International Osteoporosis Foundation suggest a treatment threshold determined by PTH response to vitamin D levels less than 30 ng/mL [57].

CONCLUSIONS

Hypovitaminosis D may have several implications in the pathophysiology and treatment of shoulder pathologies. Vitamin D has various physiological effects involved in modulating tissue healing, bone growth, and maintenance of homeostasis in skeletal muscle cells. Vitamin D also has anti-inflammatory effects that are important to rotator cuff health. Vitamin D deficiency is highly prevalent in patients with RCTs, suggesting its role as a potential risk factor. In addition to impacting preoperative shoulder strength, vitamin D deficiency may increase retear rates, postoperative stiffness, and the need for revision surgery in patients who underwent RCR. A potential association between vitamin D deficiency and increased risk of revision TSA has been demonstrated, but the reason for this finding is unclear. Further research on optimal vitamin D deficiency management in such patients is warranted to optimize postoperative outcomes of TSA and RCR.

Notes

Author contributions

Writing – original draft: MD, OC, RL, PB, MCRH, ZZ, MYF. Writing – review & editing: JAA. All authors read and agreed to the published version of the manuscript. All authors read and agreed to the published version of the manuscript.

Conflict of interest

JAA would like to disclose royalties from DJO Global, Zimmer-Biomet, Smith and Nephew, Stryker, Globus Medical, Inc.; research support as a PI from: Lima Corporation - Italy, Orthofix, Arthrex, OREF; royalties, financial or material support from Wolters Kluwer; and board member/committee appointments for American Shoulder and Elbow Society, Pacira.

Funding

None.

Data availability

None.

Acknowledgments

None.

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Table 1.

Summary of the studies assessing the impact of hypovitaminosis D on the rotator cuff

Study	Cohort	Findings
Albright et al. (2023) [34]	Population: 672, 640 (336,320 vitamin D deficient) patients undergoing RCR matched patients with vitamin D deficiency	Vitamin D deficiency risk factor for full-thickness RCT (OR, 2.36; 95% CI, 2.17–2.56). Greatest effect in women aged 70–89 (OR, 2.93) and lowest in men between 30 and 49 (OR, 1.87)
	Source: PearlDiver Humana database	Women with RCT and vitamin D deficiency more likely to undergo RCR (OR, 1.37; 95% CI, 1.09–1.74), no difference for male RCT patients with or without vitamin D deficiency
		Stiffness requiring MUA more likely in vitamin D deficient RCR patients (OR, 1.14; 95% CI, 1.03-1.27)
		Similar rates of revision, I&D for vitamin D deficiency patients (P>0.05).
Kim et al. (2023) [23]	68 Patients (12 vitamin D deficient) undergoing RCR for medium (1–3 cm) full-thickness RCT	Gene expression
	Source: single institution retrospective study	In vitamin D deficient RCR patients, deltoid VDR expression lower (0.16 vs. 0.34, P<0.05), myoD expression lower (1.09 vs. 2.84, P<0.05), atrogin expression higher (1.05 vs. 0.23, P<0.05).
		No difference in muscle-atrophy or adipogenic gene expression within supraspinatus tissue samples between vitamin D deficient versus sufficient cohorts.
		Inflammation-related genes IL-1B, IL-6 expressed more in vitamin D deficient patients’ deltoid and supraspinatus
		No difference in MMP-9, COL1A1, COL3A1, p53, TNF-α gene expression.
		Protein expression
		In deltoids of vitamin D deficient patients, protein expression of atrogin and IL-6 increased (P<0.05). In the supraspinatus, only IL-6 expression increased with vitamin D deficiency (P<0.05).
		Proinflammatory proteins upregulated in vitamin D deficient patients (P<0.05).
		Vitamin D deficiency led to expression of muscle atrophy-related proteins in the deltoid (P<0.05), but not the rotator cuff (P>0.05).
Wilde et al. (2023) [36]	135 Patients (34 with low vitamin D) undergoing RCR	36% of included RCR patients had low vitamin D.
Wilde et al. (2023) [36]	Source: Single institution retrospective study	36% of included RCR patients had low vitamin D.
Rhee et al. (2023) [4]	36 Patients undergoing RCR for full-thickness, small to massive RCTs	91.7% (33/36) insufficient or deficient vitamin D
	Source: single institution prospective study	Lower serum and tissue vitamin D correlated with preoperative and 1-year postoperative abduction strength (P<0.05).
		Tissue vitamin D correlate with preoperative, but not postoperative VAS pain, ASES, Constant scores (all P<0.05).
		Vitamin D had no relationship with fatty degeneration of the rotator cuff, tear size, retraction (P>0.05).
		Muscle fiber cross-sectional area significantly related to tissue, but not serum vitamin D levels (P<0.05).
Liu et al. (2022) [35]	104 Full-thickness RCT patients (72 with osteoporosis) undergoing RCR. Excluded obese patients.	Vitamin D3 was significantly lower in RCT-OP (16.7 ng/mL) group compared to RCT only (19.6 ng/mL) (P<0.05).
Liu et al. (2022) [35]	Source: single institution prospective study	Vitamin D predictive of RCT in osteoporotic patients (P<0.05)
Lee et al. (2021) [5]	176 Patients (78 with low vitamin D) undergoing RCR for full-thickness RCT.	71% of itamin D insufficient or deficient
	Source: single institution retrospective study	Prevalence of low vitamin D decreased with age (P<0.05).
		More likely in indoor workers (36.2 vs. 48.3%, P=0.001)
		No relationship between sex, smoking status, alcohol consumption and low vitamin D

RCR: rotator cuff repair, RCT: rotator cuff tear, OR: odds ratio, MUA: manipulation under anesthesia, I&D: irrigation and debridement, IL: interleukin, VAS: visual analog scale, ASES: American Shoulder and Elbow Surgeons, RCT-OP: RCT-osteoporosis.

Study	Cohort	Main findings
Angeline et al. 2014) [12]	Vitamin D deficiency was induced in 28 rats undergoing rotator cuff repair.	Low vitamin D levels affect the early healing after rotator cuff repair, affecting the strength at 2 weeks postoperatively.
Angeline et al. 2014) [12]		The strength was back to normal at 4 weeks postoperatively despite the histological abnormalities being still present.
Harada et al. (2019) [39]	National Claims Database	A higher rate of postoperative rotator cuff repair revision (P<0.001) and manipulation under anesthesia for stiffness (P=0.035) was seen in the vitamin D deficient cohort.
Harada et al. (2019) [39]	229 Patients with low levels of vitamin D and 1,652 patients with normal levels undergoing rotator cuff repair
Cancienne et al. (2019) [42]	PearlDiver database	A higher rate of postoperative rotator cuff repair revision was seen when the normal and deficient group were compared (P=0.007).
	199 Patients had vitamin D deficiency, 322 had vitamin D insufficiency, and 541 did not have low levels of Vitamin D.
	All of the patients underwent rotator cuff repair.
O’Donnell et al. (2020) [43]	PearlDiver database	Vitamin D deficiency (OR, 1.18; P<0.001; 95% CI, 1.08–1.28) was shown to increase the risk of revision rotator cuff repair significantly.
	41,467 Patients underwent rotator cuff repair
	3,072 Underwent revision rotator cuff repair.
Chen et al. (2022) [38]	45 Vitamin D deficient patients and 44 patients with normal levels of vitamin D	Vitamin D deficiency is not correlated with clinical function scores.
Chen et al. (2022) [38]		Vitamin D deficiency is associated with the early postoperative pain (P<0.05) and a higher retear rate (P<0.05).
Chen et al. (2022) [44]	62 Vitamin D deficient patients and 60 patients with normal levels of vitamin D	Vitamin D deficiency is not correlated with clinical function scores or retear rate.
Chen et al. (2022) [44]		Vitamin D deficiency is associated with the early postoperative pain (P<0.05) and the quality of rotator cuff healing after surgery (P=0.007).
Ryu et al. (2015) [37]	80 Vitamin D deficient patients, 8 patients with vitamin D insufficiency, and 3 patients with normal levels of vitamin D	Vitamin D deficiency had no significant impact on postoperative structural integrity and functional outcomes after arthroscopic rotator cuff repair.
Rhee et al. (2023) [4]	-	Lower serum vitamin D levels at 1 year after surgery
		weakened abduction power while tissue level of vitamin D affected both external rotation and abduction power.
		Muscle fiber cross sectional area was lower in the patients with low tissue vitamin D levels but did not correlate with serum vitamin D levels.
		Vitamin D levels did not correlate with rotator cuff repair failure.

Study	Cohort	Findings
Maier et al. (2014) [54]	Single institution in Germany: 190 patients (109 primary arthroplasty, 50 PJI, 31 aseptic loosening)^a)	All patient subgroups showed low 25(OH)D levels (<20 ng/mL)
Maier et al. (2014) [54]		PJI (13.29±6.54 ng/mL) patients had lower 25(OH)D levels compared to primary arthroplasty (19.46±9.49 ng/mL, P<0.001) and aseptic loosening (20.52±9.13 ng/mL, P<0.001).
Inkrott et al. (2016) [2]	Single institution in USA: 286 patients (2 HA, 52 aTSA, 164 rTSA)	93 (43%) 25(OH)D insufficient
		24 (11%) 25(OH)D deficient
		BMI >30 kg/m² lower 25(OH)D levels (31.5±12.1 vs. 36.2±15.7 ng/mL, P<0.01).
		Absence vitamin D and calcium supplementation was most significant risk factor for hypovitaminosis D (OR, 8.234; P<0.001).
Smith et al. (2021) [3]	PearlDiver database: 6,696 TSA patients (1,674 25(OH)D deficient, 5,022 age, sex, osteoporosis matched controls)	Higher rate of revision arthroplasty in 25(OH)D deficient compared to controls (2.3% vs. 0.8%; OR, 3.3; P<0.0001)
Smith et al. (2021) [3]		Higher risk UTI compared to controls (OR, 1.31; P=0.003)